SDD-AGE SDS-PAGE
SDS-PAGE (sodium dodecyl sulfate–polyacrylamide gel electrophoresis)
It uses sodium dodecyl sulfate (SDS) molecules to help identify and isolate protein molecules. It separate proteins with molecular masses between 5 and 250 KD.
The medium (also referred to as ′matrix′) is a polyacrylamide-based discontinuous gel. In addition, SDS (sodium dodecyl sulfate) is used. About 1.4 grams of SDS bind to a gram of protein, corresponding to one SDS molecule per two amino acids. SDS acts as a surfactant, covering the proteins’ intrinsic charge and conferring them very similar charge-to-mass ratios.
SDD-AGE is short for Semi-Denaturating Detergent Agarose Gel Electrophoresis.
Agarose is used for the gel since the SDS-resistant polymers are large (in the 200-4000+ kDa range) and cannot enter a conventional polyacrylamide gel, which has small pores. Agarose on the other hand has large pores, which allows for the separation of polymers.
| SDS-PAGE | Sodium Dodecyl Sulfate | PolyAcrylamide Gel Electrophoresis |
| 5-250kD | 十二烷基硫酸钠 | 聚丙烯酰胺凝胶电泳 |
| SDD-AGE | Semi-Denaturating Detergent | Agarose Gel Electrophoresis |
| 200-4000kD | 半变性洗涤剂 | 琼脂糖凝胶电泳 |